| City | Population |
| Alafaya, FL | 78,113 |
| Altamonte Springs, FL | 41,496 |
| Altoona, FL | 89 |
| Apopka, FL | 41,542 |
| Astatula town, FL | 1,810 |
| Astor, FL | 1,556 |
| Azalea Park, FL | 12,556 |
| Babson Park, FL | 1,356 |
| Bay Hill, FL | 4,884 |
| Bay Lake, FL | 47 |
| Belle Isle, FL | 5,988 |
| Beverly Beach town, FL | 338 |
| Bithlo, FL | 8,268 |
| Black Hammock, FL | 1,144 |
| Brevard County rural, FL | 105,424 |
| Buenaventura Lakes, FL | 26,079 |
| Bunnell, FL | 2,676 |
| Campbell, FL | 2,479 |
| Cape Canaveral, FL | 9,912 |
| Casselberry, FL | 26,241 |
| Celebration, FL | 7,427 |
| Christmas, FL | 1,146 |
| Chuluota, FL | 2,483 |
| Clarcona, FL | 2,990 |
| Clermont, FL | 28,742 |
| Cocoa, FL | 17,140 |
| Cocoa Beach, FL | 11,231 |
| Cocoa West, FL | 5,925 |
| Conway, FL | 13,467 |
| Crescent City, FL | 1,577 |
| Crooked Lake Park, FL | 1,722 |
| Davenport, FL | 2,888 |
| Daytona Beach, FL | 61,005 |
| Daytona Beach Shores, FL | 4,247 |
| De Leon Springs, FL | 2,614 |
| DeBary, FL | 19,320 |
| DeLand, FL | 27,031 |
| DeLand Southwest, FL | 1,052 |
| Deltona, FL | 85,182 |
| Doctor Phillips, FL | 10,981 |
| Eatonville town, FL | 2,159 |
| Edgewater, FL | 20,750 |
| Edgewood, FL | 2,503 |
| Eustis, FL | 18,558 |
| Fairview Shores, FL | 10,239 |
| Fellsmere, FL | 5,197 |
| Fern Park, FL | 7,704 |
| Ferndale, FL | 472 |
| Flagler Beach, FL | 4,484 |
| Flagler County rural, FL | 13,002 |
| Forest City, FL | 13,854 |
| Four Corners, FL | 26,116 |
| Fruitland Park, FL | 4,078 |
| Geneva, FL | 2,940 |
| Gifford, FL | 9,590 |
| Glencoe, FL | 2,582 |
| Goldenrod, FL | 12,039 |
| Gotha, FL | 1,915 |
| Grant-Valkaria town, FL | 3,850 |
| Groveland, FL | 8,729 |
| Haines City, FL | 20,535 |
| Heathrow, FL | 5,896 |
| Highland Park village, FL | 230 |
| Hillcrest Heights town, FL | 254 |
| Holden Heights, FL | 3,679 |
| Holly Hill, FL | 11,659 |
| Horizon West, FL | 14,000 |
| Howey-in-the-Hills town, FL | 1,098 |
| Hunters Creek, FL | 14,321 |
| Indialantic town, FL | 2,720 |
| Indian Harbour Beach, FL | 8,225 |
| Indian River County rural, FL | 23,624 |
| Indian River Shores town, FL | 3,901 |
| June Park, FL | 4,094 |
| Kissimmee, FL | 59,682 |
| Lady Lake town, FL | 13,926 |
| Lake Buena Vista, FL | 10 |
| Lake Butler, FL | 15,400 |
| Lake County rural, FL | 139,701 |
| Lake Hart, FL | 542 |
| Lake Helen, FL | 2,624 |
| Lake Kathryn, FL | 920 |
| Lake Mack-Forest Hills, FL | 1,010 |
| Lake Mary, FL | 13,822 |
| Lake Mary Jane, FL | 1,575 |
| Lake Wales, FL | 14,225 |
| Leesburg, FL | 20,117 |
| Lisbon, FL | 260 |
| Lockhart, FL | 13,060 |
| Longwood, FL | 13,657 |
| Loughman, FL | 2,680 |
| Maitland, FL | 15,751 |
| Malabar town, FL | 2,757 |
| Mascotte, FL | 5,101 |
| Meadow Woods, FL | 25,558 |
| Melbourne, FL | 76,068 |
| Melbourne Beach town, FL | 3,101 |
| Melbourne Village town, FL | 662 |
| Merritt Island, FL | 34,743 |
| Micco, FL | 9,052 |
| Midway, FL | 1,705 |
| Mims, FL | 7,058 |
| Minneola, FL | 9,403 |
| Montverde town, FL | 1,463 |
| Mount Dora, FL | 12,370 |
| Mount Plymouth, FL | 4,011 |
| New Smyrna Beach, FL | 22,464 |
| North DeLand, FL | 1,450 |
| Oak Hill, FL | 1,792 |
| Oak Ridge, FL | 22,685 |
| Oakland town, FL | 2,538 |
| Ocoee, FL | 35,579 |
| Okahumpka, FL | 267 |
| Orange City, FL | 10,599 |
| Orange County rural, FL | 304,217 |
| Orchid town, FL | 415 |
| Orlando, FL | 238,300 |
| Orlovista, FL | 6,123 |
| Ormond Beach, FL | 38,137 |
| Ormond-by-the-Sea, FL | 7,406 |
| Osceola County rural, FL | 111,479 |
| Oviedo, FL | 33,342 |
| Paisley, FL | 818 |
| Palm Bay, FL | 103,190 |
| Palm Coast, FL | 75,180 |
| Palm Shores town, FL | 900 |
| Paradise Heights, FL | 1,215 |
| Patrick AFB, FL | 1,222 |
| Pierson town, FL | 1,736 |
| Pine Castle, FL | 10,805 |
| Pine Hills, FL | 60,076 |
| Pine Lakes, FL | 862 |
| Pittman, FL | 180 |
| Pomona Park town, FL | 912 |
| Ponce Inlet town, FL | 3,032 |
| Port Orange, FL | 56,048 |
| Port St. John, FL | 12,267 |
| Rio Pinar, FL | 5,211 |
| Rockledge, FL | 24,926 |
| Roseland, FL | 1,472 |
| Samsula-Spruce Creek, FL | 5,047 |
| Sanford, FL | 53,570 |
| Satellite Beach, FL | 10,109 |
| Sebastian, FL | 21,929 |
| Seminole County rural, FL | 137,545 |
| Seville, FL | 614 |
| Sharpes, FL | 3,411 |
| Silver Lake, FL | 1,879 |
| Sky Lake, FL | 6,153 |
| Sorrento, FL | 861 |
| South Apopka, FL | 5,728 |
| South Daytona, FL | 12,252 |
| South Patrick Shores, FL | 5,875 |
| Southchase, FL | 15,921 |
| St. Cloud, FL | 35,183 |
| Taft, FL | 2,205 |
| Tangelo Park, FL | 2,231 |
| Tangerine, FL | 2,865 |
| Tavares, FL | 13,951 |
| The Villages, FL | 51,442 |
| Tildenville, FL | 511 |
| Titusville, FL | 43,761 |
| Umatilla, FL | 3,456 |
| Union Park, FL | 9,765 |
| University, FL | 31,084 |
| Vero Beach, FL | 15,220 |
| Vero Beach South, FL | 23,092 |
| Viera East, FL | 10,757 |
| Viera West, FL | 6,641 |
| Volusia County rural, FL | 92,415 |
| Wabasso, FL | 609 |
| Wabasso Beach, FL | 1,853 |
| Wedgefield, FL | 6,705 |
| Wekiwa Springs, FL | 21,998 |
| Welaka town, FL | 701 |
| West DeLand, FL | 3,535 |
| West Melbourne, FL | 18,355 |
| West Vero Corridor, FL | 7,138 |
| Williamsburg, FL | 7,646 |
| Windermere town, FL | 2,462 |
| Windsor, FL | 256 |
| Winter Beach, FL | 2,067 |
| Winter Garden, FL | 34,568 |
| Winter Park, FL | 27,852 |
| Winter Springs, FL | 33,282 |
| Yalaha, FL | 1,364 |
| Yeehaw Junction, FL | 240 |
| Zellwood, FL | 2,817 |
| | |
| Total | 3,482,945 |
