| City | Population |
| Aucilla, FL | 100 |
| Carrabelle, FL | 2,778 |
| Chattahoochee, FL | 3,652 |
| Crawfordville, FL | 3,702 |
| Day, FL | 116 |
| Gadsden County rural, FL | 27,945 |
| Greensboro town, FL | 602 |
| Greenville town, FL | 843 |
| Gretna, FL | 1,460 |
| Hamilton County rural, FL | 8,598 |
| Havana town, FL | 1,754 |
| Hosford, FL | 650 |
| Jasper, FL | 4,546 |
| Jefferson County rural, FL | 11,104 |
| Jennings town, FL | 878 |
| Lafayette County rural, FL | 7,517 |
| Lamont, FL | 178 |
| Lee town, FL | 352 |
| Leon County rural, FL | 91,133 |
| Liberty County rural, FL | 6,071 |
| Live Oak, FL | 6,850 |
| Lloyd, FL | 215 |
| Madison, FL | 2,843 |
| Madison County rural, FL | 15,186 |
| Mayo town, FL | 1,237 |
| Midway, FL | 3,004 |
| Monticello, FL | 2,506 |
| Panacea, FL | 816 |
| Perry, FL | 7,017 |
| Quincy, FL | 7,972 |
| Sneads town, FL | 1,849 |
| Sopchoppy, FL | 457 |
| St. Marks, FL | 293 |
| Suwannee County rural, FL | 33,989 |
| Tallahassee, FL | 181,376 |
| Taylor County rural, FL | 14,506 |
| Wacissa, FL | 386 |
| Wakulla County rural, FL | 25,508 |
| Waukeenah, FL | 272 |
| White Springs town, FL | 777 |
| Woodville, FL | 2,978 |
| Adel, GA | 5,334 |
| Argyle town, GA | 212 |
| Atkinson County rural, GA | 4,867 |
| Attapulgus, GA | 449 |
| Bainbridge, GA | 12,697 |
| Barwick, GA | 386 |
| Berlin, GA | 551 |
| Boston, GA | 1,315 |
| Boykin, GA | 143 |
| Brinson town, GA | 215 |
| Brooks County rural, GA | 11,442 |
| Cairo, GA | 9,607 |
| Calvary, GA | 161 |
| Camilla, GA | 5,360 |
| Cecil, GA | 286 |
| Climax, GA | 280 |
| Clinch County rural, GA | 3,689 |
| Colquitt, GA | 1,992 |
| Colquitt County rural, GA | 28,168 |
| Cook County rural, GA | 8,667 |
| Coolidge, GA | 525 |
| Damascus, GA | 254 |
| Dasher town, GA | 912 |
| Decatur County rural, GA | 14,201 |
| Donalsonville, GA | 2,650 |
| Du Pont town, GA | 120 |
| Echols County rural, GA | 2,994 |
| Ellenton town, GA | 281 |
| Fargo, GA | 321 |
| Funston, GA | 449 |
| Grady County rural, GA | 14,772 |
| Hahira, GA | 2,737 |
| Homerville, GA | 2,456 |
| Iron City town, GA | 310 |
| Lake Park, GA | 733 |
| Lakeland, GA | 3,366 |
| Lanier County rural, GA | 6,712 |
| Lowndes County rural, GA | 48,324 |
| Meigs, GA | 1,035 |
| Miller County rural, GA | 3,990 |
| Mitchell County rural, GA | 12,945 |
| Moody AFB, GA | 886 |
| Morven, GA | 565 |
| Moultrie, GA | 14,268 |
| Norman Park, GA | 972 |
| Ochlocknee town, GA | 676 |
| Pavo, GA | 627 |
| Pearson, GA | 2,117 |
| Pelham, GA | 3,898 |
| Quitman, GA | 3,850 |
| Ray City, GA | 1,090 |
| Remerton, GA | 1,123 |
| Riverside town, GA | 35 |
| Seminole County rural, GA | 5,769 |
| Sparks town, GA | 2,052 |
| Statenville, GA | 1,040 |
| Thomas County rural, GA | 22,129 |
| Thomasville, GA | 18,413 |
| Valdosta, GA | 54,518 |
| Whigham, GA | 471 |
| | |
| Total | 834,423 |
